Resting the knee is probably the best thing to do.
Thinking about it, is the pain behind the kneecap? If so you may want to check seat height as pain behind the knee is one of the indicators that your seat may be too low.
Other things to consider - is 20 miles a typical distance for you on the trail? If not then maybe you went too far. Also were you turning too big a gear? Or didn't warm up properly - I get knee twinges if I do big ring stuff before warming up properly...
If it continues I'd suggest going to the docs.
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